분회초청강연


     나노바이오

  김태형 교수 중앙대학교


     ■ Education


2013.03 – 2015.08
Postdoctoral Research Fellow
Chemistry and Chemical Biology
Rutgers, The State University of New Jersey, Piscataway, NJ, U.S.A

2008.03 – 2013.02
Ph. D.
Chemical & Biomolecular Engineering

Sogang University, Seoul, Korea
2001.03 – 2008.02
B. S.
Chemical Engineering

Sogang University, Seoul, Korea


■  Academic Posisition


2019.09 – Present
Associate Professor
2015.09 – 2019.08
Assistant Professor
Integrative Engineering (Biomedical Engineering)
Chung-Ang University, Seoul, Korea



Label-free and non-destructive characterizations of stem cell differentiation 

and organoid generation


Stem cells have emerged as valuable sources for regenerative therapy and drug screening. They can generate multiple types of cells and are capable of forming an organ-like structure in vitro that is named organoids. However, precise characterizations of the stem cell differentiation into specific lineages and organoids are still challenging without loss of cell viability and function. In this study, we report a new type of analytical platform that enables precise, non-destructive, and label-free characterizations of several key stem cell feature including pluripotency/multipotency, differentiation and organoid maturation.

The autofluorescence and Raman integration (ARMI) method was developed as an optical tool for the rapid analysis of adipogenic and osteogenic differentiation of human adipose-derived mesenchymal stem cells (hAMSCs) in vitro. The method was further advanced to a three-dimensional analysis of odontogenesis of human dental pulp stem cells (hDPSCs) spheroids based on the β-carotene and HA depositions.

Besides the optical platforms, the electrochemical approach was also developed as an efficient tool to selectively detect the undifferentiated human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). The remaining stem cells were successfully quantified using a Geltrex-modified highly conductive gold nanostructure (HCGN) platform after kidney organoid generation. The Same approach was also shown to be applicable to cancer cell research where the HCGN effective in generating multicellular cancer cell spheroids and viability detection under drug treatment conditions.

Therefore, it can be concluded that our platform is highly useful as a new label-free and non-destructive tool for stem cell characterizations, which will ultimately contribute to the development of regenerative medicine.





  박성수 교수 성균관대학교


     ■ Education


1997-1999
Ph.D.
Food Science
Cornell University (Ithaca, NY, USA)
1994-1997
M.Sc.
Food Science

Cornell, University (Ithaca, NY, USA)
1987-1994
B.Sc.
Microbiology
Pusan National University (Pusan, Korea)-military service


■  Academic Posisition


2014 – pres.

Director of BK21 Program/Department Head/Professor

Mechanical Engineering

Sungkyunkwan University (SKKU) (Suwon, Korea)

2008 - 2014
Associate/Full Professor
Chemistry and Nano Sciences
Ewha Womans University (Seoul, Korea)
2010 – 2015
Visiting Professor & Co-Principal Investigator
Mechanobiology Institute (MBI)
National University of Singapore (NUS) (Singapore)

2004 – 2008

Assistant Professor

Nano Sciences

Ewha Womans University (Seoul, Korea)

2001 – 2004

Post-doc

Physics

Princeton University (Princeton, NJ, USA)

2000 – 2001

Post-doc

Bioengineering

Cornell University (Ithaca, NY, USA)

1999 – 2000

Post-doc

Biological Information

Tokyo Institute of Technology (Tokyo, Japan)

 


Label-free and non-destructive characterizations of stem cell differentiation

and organoid generation



Stem cells have emerged as valuable sources for regenerative therapy and drug screening. They can generate multiple types of cells and are capable of forming an organ-like structure in vitro that is named organoids. However, precise characterizations of the stem cell differentiation into specific lineages and organoids are still challenging without loss of cell viability and function. In this study, we report a new type of analytical platform that enables precise, non-destructive, and label-free characterizations of several key stem cell feature including pluripotency/multipotency, differentiation and organoid maturation.

The autofluorescence and Raman integration (ARMI) method was developed as an optical tool for the rapid analysis of adipogenic and osteogenic differentiation of human adipose-derived mesenchymal stem cells (hAMSCs) in vitro. The method was further advanced to a three-dimensional analysis of odontogenesis of human dental pulp stem cells (hDPSCs) spheroids based on the β-carotene and HA depositions.

Besides the optical platforms, the electrochemical approach was also developed as an efficient tool to selectively detect the undifferentiated human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). The remaining stem cells were successfully quantified using a Geltrex-modified highly conductive gold nanostructure (HCGN) platform after kidney organoid generation. The Same approach was also shown to be applicable to cancer cell research where the HCGN effective in generating multicellular cancer cell spheroids and viability detection under drug treatment conditions.

Therefore, it can be concluded that our platform is highly useful as a new label-free and non-destructive tool for stem cell characterizations, which will ultimately contribute to the development of regenerative medicine.



  송대섭 교수 고려대학교


     ■ Education


2004

Doctor of Veterinary Medicine

Seoul National University, Seoul, Korea

2000Doctor of Philosophy
Veterinary Microbiology. Seoul National University, Seoul, Korea








■  Professional Record


 2020 - Present
Professor
Korea University

 2016 - 2017

General director

The Korean Vaccine Society

 2016 - 2017

Finance director

Korea Society for Zoonoses

2015 - 2020
Associate Professor
Korea University
2015Member of MERS Joint Korea/WHO mission team

2011 - 2015

Adjunct Associate Professor

University of Science and Technology

2010 - 2015

Senior Researcher

Korea Research Institute of Bioscience and Biotechnology

2006 – 2010

Senior Researcher. Research unit

Green Cross Veterinary Products, Yongin, Korea

2004 – 2006

Researcher. Research unit

Green Cross Veterinary Products, Yongin, Korea

 


Nanobiotechnology for diagnosis, vaccine and treatment against Disease X

; unexpected emerging viral diseases



Unlike in the past, emergences of viral infections have been and are occurring at very frequent intervals, causing enormous deaths and disability worldwide. Against a constant background of established infections, periodical emerging the epidemics of highly pathogenic influenza viruses (HPAI) greatly expand the global burden of infections. Accurate and rapid diagnosis of viral infections can result in effective and appropriate prevention and quarantine measures. This study compares and discusses about rapid diagnostics for the differential patho-typing between HPAI and LPAI using nanobiotechnology. The field of nanotechnology encompasses those technologies to fabricate materials, including sphere, cubic and nanoscale particles. Therefore, nanobiotechnology has the potential to offer not merely advances in diagnostics and vaccination to control infectious diseases but also in delivering various capabilities as below; Highly pathogenic avian influenza virus (HPAIV) infections have occurred continuously and crossed the species barrier to humans, leading to fatalities. A PCR-based molecular test is currently the most sensitive diagnostic tool for HPAIV; however, the results must be analyzed in centralized diagnosis systems by a trained individual. This requirement leads to delays in quarantine and isolation. To control the spread of HPAIV, rapid and accurate diagnostics suitable for field testing are needed, and the tests must facilitate a differential diagnosis between HPAIV and low pathogenic avian influenza virus (LPAIV), which undergo cleavage specifically by trypsin- or furin-like proteases, respectively. In this study, we have developed a differential avian influenza virus (AIV) rapid test kit and evaluated it in vitro and using clinical specimens from HPAIV H5N1-infected dogs. We demonstrated that this rapid test kit provides highly sensitive and specific detection of HPAIV and LPAIV and is thus a useful field diagnostic tool for H5N1 HPAIV outbreaks and for rapid quarantine control of the disease. In addition to diagnostics, development of better adjuvant accompanied with vaccine for enhancing immunogenicity has been greatly required for the control of influenza infection. Herein, we also address nano-complex of amphiphilic grafted poly (amino acid) and hydrophobic squalene (PA/S-NC) as a potent adjuvant that can act as a robust strategy for induce humoral (Th2) and cellular (Th1) immune responses as well as a delivery agent of antigens. CASq performed great biocompatibility, particle stability, and produced a high degree of antigen-specific antibodies and T cell immune responses in mice when CASq was co-administered with inactivated whole influenza virus antigen (CA04), in which CASq exhibited complete protection against lethal infection.

Keyword: HPAIV, nanobiotechnology, diagnostics, adjuvant, vaccination



  김중배 교수 고려대학교


     ■ Education


1995

Ph.D.

Biochemical Engineering

University of Iowa, Iowa City, Iowa

1988

M.S.

Chemical Engineering

Seoul National University, Seoul, Korea

1986

B.S.

Chemical Engineering

Seoul National University, Seoul, Korea



■  Professional Record


2007-Present

Professor

Department of Chemical & Biological Engineering

Korea University, Seoul, Korea

2000-2007

Senior Research Scientist

Environmental Technology Division

Pacific Northwest National Laboratory, Richland, Washington

1998-2000

Assistant Research Scientist

Chemical Engineering

Rensselaer Polytechnic Institute, Troy, New York

 


Nanobiocatalysis and its potential applications



Enzymes are biomolecules that can catalyze a variety of chemical reactions. However, their practical applications are often hampered by their poor stability. Nanobiocatalysis, immobilizing enzymes using various nanomaterials, has demonstrated its successes in stabilizing the enzyme activity for various enzyme applications such as biosensors, biofuel cells, enzyme-linked immunosorbent assay, membrane antifouling, and CO2 conversion. This presentation will cover nanobiocatalytic stabilization of acylase and carbonic anhydrase for their successful uses in enzyme-catalyzed quorum quenching and CO2 conversion, respectively. Especially, the stabilization of carbonic anhydrase has made an unprecedented success by maintaining 83% of initial enzyme activity even after incubation in aqueous solution under shaking at 200 rpm for two years. Stabilized carbonic anhydrase was successfully employed for the effective conversion of CO2 to bicarbonate, which was further used for expedited microalgae growth and improved calcium carbonate production. If time permits, several other examples of nanobiocatalytic stabilization and applications will also be introduced.



  이성운 대표 (주)레보스케치


     ■ Education


1992 – 1996
KAIST, Korean Advanced Institute of Science and Technology.
1993~1996

EE (Electric and Electronics Engineering)

Main designer for KITSAT-3(3rd space satellite of Korea



■  Work History


2017.1 ~ Present
Revosketch Inc. , CEO
Digital PCR, molecular diagnosis solution
Currently completed Series A
2007.10 - 2016.12
3DISC , CEO
Medical Computed Radiography(CR)  Scanner
(Laser fluorescence base X-ray imager)
Max revenue: 18M USD (14M oversea, 4M domestic) @2015
Max production: 2500+/year @2015
Global Medical Regulatory including FDA/CGMP
2 oversea offices (US and Denmark)
2000.1 - 2007.10
Star V-ray, CTO
Medical/Industrial X-ray Detector
Bio Microarray Scanner (co-work with Macrogen(KR))
Ultra low noise X-ray film scanning head (co-work with Vidar(US))
Acquired by Vidar(US)
1994.1 - 1999.12
AKCRON, CEO
Satellite, KITSAT-3 (co-work with SaTrec(KR))
Satellite Tracking Antenna (co-work with ETRI(KR))
Semiconductor AOI(Auto Optical Inspector)
Merged by Photon Dynamics(US)

 


Next Generation Real-time Digital PCR



 중합효소연쇄반응(PCR; Polymerase Chain Reaction)은 1984년 발명된 이후에 발전을 거듭하여 현재 3세대 Digital PCR 기술로 빠르게 발전해 왔다. 기존 기술에 비해 비약적인 민감도 성능 개선이 이루어졌지만 3~4개 장비로 이루어지고 증폭 후에 한번만 형광을 측정하는 End-point Scan을 사용함으로써 사용이 불편하며 결과가 부정확해지는 문제를 가지고 있는 상태이다. 이에 RevoSketch는 하나의 Digital PCR 기기에 필요한 모든 기능을 통합하였으며 최초로 증폭 주기마다 스캔이 가능한 Real-time Digital PCR 기술을 개발하였다. 또한, 원심력을 이용한 자동 분주 구조와 Nano-imprinting 기법을 이용한 경제적인 소모품 생산 기술을 개발하였다. 결론적으로, 비전문가의 편리한 사용이 가능한 고 민감도 성능을 제공하는 동시에 대량 생산이 가능한 저비용 소모품 구조로 경제적인 Digital PCR기술을 제공 한다.